Department of
Biomedical Engineering
BIOCHEMISTRY AND
HUMAN PHYSIOLOGY LAB
VIVA VOCE
QUESTIONS
- Biological
importance of Carbohydrate
- What
is the difference between reducing and non reducing sugar?
- What
is the confirmation test for monosaccharide?
- What
is the significance of osazone?
- Biological
importance of Lipids
- Difference
between essential and nonessential amino acids
- Give
eg for semi essential amino acids
- What
is meant by Partition coefficient?
- Basic principle of Chromatography
- What is the choice of stationary phase and mobile phase in
- Basic
principle of Electrophoresis
- What
is the normal value of hemoglobin in women?
- List
out the normal value of Lipid profile
- List
out the risk factor for atherosclerosis
- What is meant PCV?
- What
is meant by MCH, MCV?
- What
is the role PMNL?
- What is the level of fasting blood glucose?
- What
is the difference between ketose and aldose sugar?
- What
is the use of blank in colorimetric estimation?
- How
to prepare a 4N solution?
- What is normality, molality, molarity?
- Define
Beer – Lamberts law
- Give
an eg for basic amino acids
- What
is the normal concentration of blood cholesterol?
- What is meant by oligosaccharide ? give eg
- What is meant by polysaccharide ? give eg
- What is meant by monosaccharide ? give eg
- What is meant by ketogenic amino
acid ?
- What
is meant by glucogenic amino acid ?
- Give examples for glucogenic and
ketogenic aminoacid
- classification of carbohydrates
- classification
of Lipids
- classification
of Proteins
- classification of amino acids
- lipoprotein
classification and its function
- Define enzyme
- Define
active site
- Define
specificity of enzymes
- Give
examples for allosteric regulation of enzymes
- Give
examples for covalent modification of enzymes
- Give examples for feed back
regulation of enzymes
- Give examples for hormonal
regulation of enzymes
- List
out the clinical significances of SGPT
- List out the clinical
significances of SGOT
- List
out the clinical significances of CPK
- List out the clinical significances of LDH
- Define electrophoresis
- Define
isoelectric foccusing
- What
is meant by PAGE?
- Define
chromatography
- What
is meant by partition coefficient?
- What
is the role of mobile and stationary phase in chromatography?
- Give
the principle of adsorption
- Principle
and application of gel
electrophoresis
- Give
the normal value of the following
a) Blood Glucose
b) Total Protein
c) Serum Albumin, Globulin,
Fibrinogen
d) Albumin to globulin ratio
e) Total Cholesterol, LDL, HDL
f) Lipid Profile
g) Urine Creatinine
h) Haemoglobin
i) Erythrocyte sedimentation rate
j) PCV, MCH, MCV, MCHC, CI
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